Construction and characterization of an infectious vaccinia virus recombinant that expresses the influenza hemagglutinin gene and induces resistance to influenza virus infection in hamsters.
Identifieur interne : 002568 ( Main/Exploration ); précédent : 002567; suivant : 002569Construction and characterization of an infectious vaccinia virus recombinant that expresses the influenza hemagglutinin gene and induces resistance to influenza virus infection in hamsters.
Auteurs : G L Smith ; B R Murphy ; B. MossSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 1983.
Descripteurs français
- KwdFr :
- ADN recombiné (métabolisme), ADN viral (génétique), Animaux, Cellules HeLa, Cricetinae, Grippe humaine (immunologie), Gènes viraux, Humains, Hémagglutinines virales (génétique), Immunité innée, Mesocricetus, Méthode des plages virales, Production d'anticorps, Transfection, Virus de la grippe A (génétique), Virus de la grippe A (immunologie), Virus de la grippe A (pathogénicité), Virus de la vaccine (génétique), Virus de la vaccine (immunologie).
- MESH :
- génétique : ADN viral, Hémagglutinines virales, Virus de la grippe A, Virus de la vaccine.
- immunologie : Grippe humaine, Virus de la grippe A, Virus de la vaccine.
- métabolisme : ADN recombiné.
- pathogénicité : Virus de la grippe A.
- Animaux, Cellules HeLa, Cricetinae, Gènes viraux, Humains, Immunité innée, Mesocricetus, Méthode des plages virales, Production d'anticorps, Transfection.
English descriptors
- KwdEn :
- Animals, Antibody Formation, Cricetinae, DNA, Recombinant (metabolism), DNA, Viral (genetics), Genes, Viral, HeLa Cells, Hemagglutinins, Viral (genetics), Humans, Immunity, Innate, Influenza A virus (genetics), Influenza A virus (immunology), Influenza A virus (pathogenicity), Influenza, Human (immunology), Mesocricetus, Transfection, Vaccinia virus (genetics), Vaccinia virus (immunology), Viral Plaque Assay.
- MESH :
- chemical , genetics : DNA, Viral, Hemagglutinins, Viral.
- chemical , metabolism : DNA, Recombinant.
- genetics : Influenza A virus, Vaccinia virus.
- immunology : Influenza A virus, Influenza, Human, Vaccinia virus.
- pathogenicity : Influenza A virus.
- Animals, Antibody Formation, Cricetinae, Genes, Viral, HeLa Cells, Humans, Immunity, Innate, Mesocricetus, Transfection, Viral Plaque Assay.
Abstract
A DNA copy of the influenza virus hemagglutinin gene, derived from influenza virus A/Jap/305/57 (H2N2) was inserted into the genome of vaccinia virus under the control of an early vaccinia virus promoter. Tissue culture cells infected with the purified recombinant virus synthesized influenza hemagglutinin, which was glycosylated and transported to the cell surface where it could be cleaved with trypsin into HA1 and HA2 subunits. Rabbits and hamsters inoculated intradermally with recombinant virus produced circulating antibodies that inhibited hemagglutination by influenza virus. Furthermore, vaccinated hamsters achieved levels of antibody similar to those obtained upon primary infection with influenza virus and were protected against respiratory infection with the A/Jap/305/57 influenza virus.
DOI: 10.1073/pnas.80.23.7155
PubMed: 6580632
Affiliations:
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Le document en format XML
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Moss</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><imprint><date when="1983" type="published">1983</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibody Formation</term><term>Cricetinae</term><term>DNA, Recombinant (metabolism)</term><term>DNA, Viral (genetics)</term><term>Genes, Viral</term><term>HeLa Cells</term><term>Hemagglutinins, Viral (genetics)</term><term>Humans</term><term>Immunity, Innate</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (immunology)</term><term>Influenza A virus (pathogenicity)</term><term>Influenza, Human (immunology)</term><term>Mesocricetus</term><term>Transfection</term><term>Vaccinia virus (genetics)</term><term>Vaccinia virus (immunology)</term><term>Viral Plaque Assay</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN recombiné (métabolisme)</term><term>ADN viral (génétique)</term><term>Animaux</term><term>Cellules HeLa</term><term>Cricetinae</term><term>Grippe humaine (immunologie)</term><term>Gènes viraux</term><term>Humains</term><term>Hémagglutinines virales (génétique)</term><term>Immunité innée</term><term>Mesocricetus</term><term>Méthode des plages virales</term><term>Production d'anticorps</term><term>Transfection</term><term>Virus de la grippe A (génétique)</term><term>Virus de la grippe A (immunologie)</term><term>Virus de la grippe A (pathogénicité)</term><term>Virus de la vaccine (génétique)</term><term>Virus de la vaccine (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term><term>Hemagglutinins, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA, Recombinant</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term><term>Vaccinia virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term><term>Hémagglutinines virales</term><term>Virus de la grippe A</term><term>Virus de la vaccine</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Grippe humaine</term><term>Virus de la grippe A</term><term>Virus de la vaccine</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term><term>Influenza, Human</term><term>Vaccinia virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN recombiné</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Antibody Formation</term><term>Cricetinae</term><term>Genes, Viral</term><term>HeLa Cells</term><term>Humans</term><term>Immunity, Innate</term><term>Mesocricetus</term><term>Transfection</term><term>Viral Plaque Assay</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules HeLa</term><term>Cricetinae</term><term>Gènes viraux</term><term>Humains</term><term>Immunité innée</term><term>Mesocricetus</term><term>Méthode des plages virales</term><term>Production d'anticorps</term><term>Transfection</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A DNA copy of the influenza virus hemagglutinin gene, derived from influenza virus A/Jap/305/57 (H2N2) was inserted into the genome of vaccinia virus under the control of an early vaccinia virus promoter. Tissue culture cells infected with the purified recombinant virus synthesized influenza hemagglutinin, which was glycosylated and transported to the cell surface where it could be cleaved with trypsin into HA1 and HA2 subunits. Rabbits and hamsters inoculated intradermally with recombinant virus produced circulating antibodies that inhibited hemagglutination by influenza virus. Furthermore, vaccinated hamsters achieved levels of antibody similar to those obtained upon primary infection with influenza virus and were protected against respiratory infection with the A/Jap/305/57 influenza virus.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Moss, B" sort="Moss, B" uniqKey="Moss B" first="B" last="Moss">B. Moss</name><name sortKey="Murphy, B R" sort="Murphy, B R" uniqKey="Murphy B" first="B R" last="Murphy">B R Murphy</name><name sortKey="Smith, G L" sort="Smith, G L" uniqKey="Smith G" first="G L" last="Smith">G L Smith</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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